Central Valley's and Sierra's varsity football programs have faced off eight times since 2006.
The Hawks lost the latest matchup, 20-14, to the Wolves Friday evening at Bulldog Stadium.
Trailing by six points at Sierra's 7-yard line with 10 seconds to play, Central Valley quarterback Miguel Jimenez's potential game-tying pass on fourth down was intercepted in the end zone by Wolves' defensive back Larenz Redd.
Redd's timely pick preserved the hard-fought victory.
"We found ways to lose the game throughout the night," Hawks head coach Scott Edwards said. "It wasn't just one play. All the mistakes added up. The greatest learning takes place from failures. Hopefully, this ignites a good reaction."
Central Valley (1-1) has never beaten Sierra (1-1).
The Wolves rallied for a 28-26 victory last season.
Sierra topped Central Valley 23-13 in 2015, 56-0 in 2014, 49-20 in 2009, 31-23 in 2008, 32-24 in 2007 and 41-21 in 2006.
The Hawks are 0-4 versus the Wolves under Edwards' guidance.
"We wanted to make history by beating Sierra for the first time," Central Valley third-year lineman David Serrano said. "It's disappointing we didn't win. We prepared the whole week. We had trouble executing. Little things killed us like turnovers, penalties and missed tackles."
"This one hurts," Edwards said. "The difference comes down to confidence. As times got tense, we lost focus. We put difficult situations on ourselves by making mental mistakes. The kids played hard and physical. They gave a great effort."
Jimenez's 33-yard touchdown pass to James Murphy and a 2-point conversion evened the score at 14-14 with 5:33 remaining in the third quarter.
Nick Stanley connected with David Reyes on a 60-yard scoring play to give Sierra a 20-14 lead with 1:49 to play.
The Hawks trailed 14-6 at intermission.
Central Valley's Ryan Palecek scored on a 3-yard run in the first quarter.
Sierra's Daemon Duran scampered 74 and 54 yards for touchdowns in the opening half.
The Hawks had a chance to tie and/or possibly win the non-league contest in the waning seconds.
A false-start penalty moved the ball back to the Wolves' 7-yard line.
Sierra stopped Central Valley on the final play of the game.
"We were going to punch it in from the 2-yard line with a run," Serrano said. "Once we got pushed back, we changed our play call. We can't let this loss hang over our heads. We have to keep working hard."
The Hawks reeled off a program-record eight straight victories after losing to Sierra last fall.
Central Valley will travel to Downey High School this Friday to face Grace Davis (2-0).
Kickoff slated for 7 p.m.
The Hawks defeated the Tim Garcia-led Spartans 34-7 in 2016.
Garcia was Central Valley's founding head coach. He directed the Hawks for six seasons (2006-11).
"We have to prepare to our fullest ability and not let our egos get ahead of us," Serrano said.
Manteca Bulletin contributed to this report.